关键词: 益生菌, 大鼠, 安全性, 毒性, 28 d 经口毒性研究

Abstract: Objective The 28 days oral toxicity of mixed Lactobacter freeze-dried powder ( zhang +R9639+P9+V9+P-8+XLTG11) in rats was studied to provide safety evidence for its application. Method The mixed freeze-dried powder were given to male and female rats intragastrically for 28 days according to the method of GB 15193. 22— 2014. The general status, body weights, organ indexes, food consumption, hematology, blood biochemistry, urinalysis, and histopathological examination of the rats were recorded and evaluated. Result Compared with the control group, no significant differences were found in general status, organ indexes, food consumption, hematology, blood biochemistry, urinalysis, and histopathological examination in rats administrated with different doses of mixed Lactobacter powder. Conclusion Theses result showed that the mixed Lactobacter freeze-dried powder had no significant toxicity in the 28 days oral toxicity assay.

Key words: probiotics, rat, safety, toxicity, 28 days oral toxicity test